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rh spp1  (R&D Systems)


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    Structured Review

    R&D Systems rh spp1
    (A) CCL20 protein levels in 786-O RCC cells after induction of EMT by TGFb. (B) DGE between macrophages within 50um of sRCC cells (right) vs >50um (left) from CosMx analysis. (C) Box plot of average <t>SPP1</t> expression of macrophages in Clear Cell, Transition, or Sarcomatoid FOVs from CosMx analysis. (D) Macrophage differentiation marker and SPP1 protein expression in polarized THP-1 derived macrophages and M0 macrophages treated with recombinant CCL20. (E) EMT protein markers from 786-O cells treated with recombinant SPP1. (F) EMT markers in 786-O cells treated with conditioned media from M0, M1, or M2-like macrophages. (G) EMT marker proteins and several proteins found to be highly differentially expressed in Sarcomatoid cells at the mRNA level (CD44, Met, ANXA2) in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (H) PD-L1 protein expression in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (I) Cell invasion assay representative images and quantification in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (J) Schematic of proposed mechanistic pathway of sarcomatoid transformation. Clear cells undergo transformation to “transition” phenotype in which they express increased cytokines including CCL20 which attract immune infiltrate including macrophages. CCL20 and other cytokines lead to M2-like polarization of macrophages with increased SPP1 expression. SPP1+ macrophages then feedback to tumor cells, inducing EMT and PD-L1 expression and leading to full sarcomatoid-like changes.
    Rh Spp1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rh+spp1/bio_rxiv__2025__06__12__656421-327-8-16?v=R%26D+Systems
    Average 95 stars, based on 54 article reviews
    rh spp1 - by Bioz Stars, 2026-07
    95/100 stars

    Images

    1) Product Images from "Spatial analysis reveals a novel inflammatory tumor transition state which promotes a macrophage-driven induction of sarcomatoid renal cell carcinoma"

    Article Title: Spatial analysis reveals a novel inflammatory tumor transition state which promotes a macrophage-driven induction of sarcomatoid renal cell carcinoma

    Journal: bioRxiv

    doi: 10.1101/2025.06.12.656421

    (A) CCL20 protein levels in 786-O RCC cells after induction of EMT by TGFb. (B) DGE between macrophages within 50um of sRCC cells (right) vs >50um (left) from CosMx analysis. (C) Box plot of average SPP1 expression of macrophages in Clear Cell, Transition, or Sarcomatoid FOVs from CosMx analysis. (D) Macrophage differentiation marker and SPP1 protein expression in polarized THP-1 derived macrophages and M0 macrophages treated with recombinant CCL20. (E) EMT protein markers from 786-O cells treated with recombinant SPP1. (F) EMT markers in 786-O cells treated with conditioned media from M0, M1, or M2-like macrophages. (G) EMT marker proteins and several proteins found to be highly differentially expressed in Sarcomatoid cells at the mRNA level (CD44, Met, ANXA2) in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (H) PD-L1 protein expression in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (I) Cell invasion assay representative images and quantification in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (J) Schematic of proposed mechanistic pathway of sarcomatoid transformation. Clear cells undergo transformation to “transition” phenotype in which they express increased cytokines including CCL20 which attract immune infiltrate including macrophages. CCL20 and other cytokines lead to M2-like polarization of macrophages with increased SPP1 expression. SPP1+ macrophages then feedback to tumor cells, inducing EMT and PD-L1 expression and leading to full sarcomatoid-like changes.
    Figure Legend Snippet: (A) CCL20 protein levels in 786-O RCC cells after induction of EMT by TGFb. (B) DGE between macrophages within 50um of sRCC cells (right) vs >50um (left) from CosMx analysis. (C) Box plot of average SPP1 expression of macrophages in Clear Cell, Transition, or Sarcomatoid FOVs from CosMx analysis. (D) Macrophage differentiation marker and SPP1 protein expression in polarized THP-1 derived macrophages and M0 macrophages treated with recombinant CCL20. (E) EMT protein markers from 786-O cells treated with recombinant SPP1. (F) EMT markers in 786-O cells treated with conditioned media from M0, M1, or M2-like macrophages. (G) EMT marker proteins and several proteins found to be highly differentially expressed in Sarcomatoid cells at the mRNA level (CD44, Met, ANXA2) in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (H) PD-L1 protein expression in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (I) Cell invasion assay representative images and quantification in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (J) Schematic of proposed mechanistic pathway of sarcomatoid transformation. Clear cells undergo transformation to “transition” phenotype in which they express increased cytokines including CCL20 which attract immune infiltrate including macrophages. CCL20 and other cytokines lead to M2-like polarization of macrophages with increased SPP1 expression. SPP1+ macrophages then feedback to tumor cells, inducing EMT and PD-L1 expression and leading to full sarcomatoid-like changes.

    Techniques Used: Expressing, Marker, Derivative Assay, Recombinant, Knockdown, Invasion Assay, Transformation Assay

    Western blot showing EMT markers in UOK-127 cells treated with 50ng/mL recombinant SPP1 at varying timepoints.
    Figure Legend Snippet: Western blot showing EMT markers in UOK-127 cells treated with 50ng/mL recombinant SPP1 at varying timepoints.

    Techniques Used: Western Blot, Recombinant

    Western blot showing SPP1 expression in THP-1 derived M2-like macrophages including control, and treatment with silencing mRNAs siNC, siPC, siSPP1#1, and siSPP1#2.
    Figure Legend Snippet: Western blot showing SPP1 expression in THP-1 derived M2-like macrophages including control, and treatment with silencing mRNAs siNC, siPC, siSPP1#1, and siSPP1#2.

    Techniques Used: Western Blot, Expressing, Derivative Assay, Control

    Western blot showing sarcomatoid-specific proteins (Met, CD44, ANXA2) and EMT markers (N-cadherin, Slug, Snail) in UOK-127 cells control, treated with M2-like macrophages, or treated with M2-like macrophages with siRNA knockdown of negative control or SPP1.
    Figure Legend Snippet: Western blot showing sarcomatoid-specific proteins (Met, CD44, ANXA2) and EMT markers (N-cadherin, Slug, Snail) in UOK-127 cells control, treated with M2-like macrophages, or treated with M2-like macrophages with siRNA knockdown of negative control or SPP1.

    Techniques Used: Western Blot, Control, Knockdown, Negative Control

    Invasion assay of UOK-127 cell co-cultured with M2 macrophage conditioned media with negative controle (siNC) or silencing of SPP1 (siSPP1) in M2 macrophages.
    Figure Legend Snippet: Invasion assay of UOK-127 cell co-cultured with M2 macrophage conditioned media with negative controle (siNC) or silencing of SPP1 (siSPP1) in M2 macrophages.

    Techniques Used: Invasion Assay, Cell Culture



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    rh spp1  (R&D Systems)
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    R&D Systems rh spp1
    (A) CCL20 protein levels in 786-O RCC cells after induction of EMT by TGFb. (B) DGE between macrophages within 50um of sRCC cells (right) vs >50um (left) from CosMx analysis. (C) Box plot of average <t>SPP1</t> expression of macrophages in Clear Cell, Transition, or Sarcomatoid FOVs from CosMx analysis. (D) Macrophage differentiation marker and SPP1 protein expression in polarized THP-1 derived macrophages and M0 macrophages treated with recombinant CCL20. (E) EMT protein markers from 786-O cells treated with recombinant SPP1. (F) EMT markers in 786-O cells treated with conditioned media from M0, M1, or M2-like macrophages. (G) EMT marker proteins and several proteins found to be highly differentially expressed in Sarcomatoid cells at the mRNA level (CD44, Met, ANXA2) in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (H) PD-L1 protein expression in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (I) Cell invasion assay representative images and quantification in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (J) Schematic of proposed mechanistic pathway of sarcomatoid transformation. Clear cells undergo transformation to “transition” phenotype in which they express increased cytokines including CCL20 which attract immune infiltrate including macrophages. CCL20 and other cytokines lead to M2-like polarization of macrophages with increased SPP1 expression. SPP1+ macrophages then feedback to tumor cells, inducing EMT and PD-L1 expression and leading to full sarcomatoid-like changes.
    Rh Spp1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rh+spp1/bio_rxiv__2025__06__12__656421-327-8-16?v=R%26D+Systems
    Average 95 stars, based on 1 article reviews
    rh spp1 - by Bioz Stars, 2026-07
    95/100 stars
    		  Buy from Supplier
    rh spp1  (MedChemExpress)
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    MedChemExpress rh spp1
    (A) CCL20 protein levels in 786-O RCC cells after induction of EMT by TGFb. (B) DGE between macrophages within 50um of sRCC cells (right) vs >50um (left) from CosMx analysis. (C) Box plot of average <t>SPP1</t> expression of macrophages in Clear Cell, Transition, or Sarcomatoid FOVs from CosMx analysis. (D) Macrophage differentiation marker and SPP1 protein expression in polarized THP-1 derived macrophages and M0 macrophages treated with recombinant CCL20. (E) EMT protein markers from 786-O cells treated with recombinant SPP1. (F) EMT markers in 786-O cells treated with conditioned media from M0, M1, or M2-like macrophages. (G) EMT marker proteins and several proteins found to be highly differentially expressed in Sarcomatoid cells at the mRNA level (CD44, Met, ANXA2) in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (H) PD-L1 protein expression in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (I) Cell invasion assay representative images and quantification in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (J) Schematic of proposed mechanistic pathway of sarcomatoid transformation. Clear cells undergo transformation to “transition” phenotype in which they express increased cytokines including CCL20 which attract immune infiltrate including macrophages. CCL20 and other cytokines lead to M2-like polarization of macrophages with increased SPP1 expression. SPP1+ macrophages then feedback to tumor cells, inducing EMT and PD-L1 expression and leading to full sarcomatoid-like changes.
    Rh Spp1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rh+spp1/pm38648200-321-7-9?v=MedChemExpress
    Average 86 stars, based on 1 article reviews
    rh spp1 - by Bioz Stars, 2026-07
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) CCL20 protein levels in 786-O RCC cells after induction of EMT by TGFb. (B) DGE between macrophages within 50um of sRCC cells (right) vs >50um (left) from CosMx analysis. (C) Box plot of average SPP1 expression of macrophages in Clear Cell, Transition, or Sarcomatoid FOVs from CosMx analysis. (D) Macrophage differentiation marker and SPP1 protein expression in polarized THP-1 derived macrophages and M0 macrophages treated with recombinant CCL20. (E) EMT protein markers from 786-O cells treated with recombinant SPP1. (F) EMT markers in 786-O cells treated with conditioned media from M0, M1, or M2-like macrophages. (G) EMT marker proteins and several proteins found to be highly differentially expressed in Sarcomatoid cells at the mRNA level (CD44, Met, ANXA2) in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (H) PD-L1 protein expression in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (I) Cell invasion assay representative images and quantification in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (J) Schematic of proposed mechanistic pathway of sarcomatoid transformation. Clear cells undergo transformation to “transition” phenotype in which they express increased cytokines including CCL20 which attract immune infiltrate including macrophages. CCL20 and other cytokines lead to M2-like polarization of macrophages with increased SPP1 expression. SPP1+ macrophages then feedback to tumor cells, inducing EMT and PD-L1 expression and leading to full sarcomatoid-like changes.

    Journal: bioRxiv

    Article Title: Spatial analysis reveals a novel inflammatory tumor transition state which promotes a macrophage-driven induction of sarcomatoid renal cell carcinoma

    doi: 10.1101/2025.06.12.656421

    Figure Lengend Snippet: (A) CCL20 protein levels in 786-O RCC cells after induction of EMT by TGFb. (B) DGE between macrophages within 50um of sRCC cells (right) vs >50um (left) from CosMx analysis. (C) Box plot of average SPP1 expression of macrophages in Clear Cell, Transition, or Sarcomatoid FOVs from CosMx analysis. (D) Macrophage differentiation marker and SPP1 protein expression in polarized THP-1 derived macrophages and M0 macrophages treated with recombinant CCL20. (E) EMT protein markers from 786-O cells treated with recombinant SPP1. (F) EMT markers in 786-O cells treated with conditioned media from M0, M1, or M2-like macrophages. (G) EMT marker proteins and several proteins found to be highly differentially expressed in Sarcomatoid cells at the mRNA level (CD44, Met, ANXA2) in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (H) PD-L1 protein expression in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (I) Cell invasion assay representative images and quantification in 786-O cells treated with conditioned media from M2 macrophages with or without SPP1 knockdown by siRNA. (J) Schematic of proposed mechanistic pathway of sarcomatoid transformation. Clear cells undergo transformation to “transition” phenotype in which they express increased cytokines including CCL20 which attract immune infiltrate including macrophages. CCL20 and other cytokines lead to M2-like polarization of macrophages with increased SPP1 expression. SPP1+ macrophages then feedback to tumor cells, inducing EMT and PD-L1 expression and leading to full sarcomatoid-like changes.

    Article Snippet: We purchased Recombinant Human (rh) TGF-beta 1 (7754-BH), rh SPP1 (1433-OP), and rh CCL20 (360-MP) from R&D systems, rh Interleukin-4 (IL-4) (ab155733) from Abcam, lipopolysaccharide (LPS) (L4391) from Sigma-Aldrich, and rh Interferon (IFN)-γ (PHC4031) from Thermo Fisher Scientific.

    Techniques: Expressing, Marker, Derivative Assay, Recombinant, Knockdown, Invasion Assay, Transformation Assay

    Western blot showing EMT markers in UOK-127 cells treated with 50ng/mL recombinant SPP1 at varying timepoints.

    Journal: bioRxiv

    Article Title: Spatial analysis reveals a novel inflammatory tumor transition state which promotes a macrophage-driven induction of sarcomatoid renal cell carcinoma

    doi: 10.1101/2025.06.12.656421

    Figure Lengend Snippet: Western blot showing EMT markers in UOK-127 cells treated with 50ng/mL recombinant SPP1 at varying timepoints.

    Article Snippet: We purchased Recombinant Human (rh) TGF-beta 1 (7754-BH), rh SPP1 (1433-OP), and rh CCL20 (360-MP) from R&D systems, rh Interleukin-4 (IL-4) (ab155733) from Abcam, lipopolysaccharide (LPS) (L4391) from Sigma-Aldrich, and rh Interferon (IFN)-γ (PHC4031) from Thermo Fisher Scientific.

    Techniques: Western Blot, Recombinant

    Western blot showing SPP1 expression in THP-1 derived M2-like macrophages including control, and treatment with silencing mRNAs siNC, siPC, siSPP1#1, and siSPP1#2.

    Journal: bioRxiv

    Article Title: Spatial analysis reveals a novel inflammatory tumor transition state which promotes a macrophage-driven induction of sarcomatoid renal cell carcinoma

    doi: 10.1101/2025.06.12.656421

    Figure Lengend Snippet: Western blot showing SPP1 expression in THP-1 derived M2-like macrophages including control, and treatment with silencing mRNAs siNC, siPC, siSPP1#1, and siSPP1#2.

    Article Snippet: We purchased Recombinant Human (rh) TGF-beta 1 (7754-BH), rh SPP1 (1433-OP), and rh CCL20 (360-MP) from R&D systems, rh Interleukin-4 (IL-4) (ab155733) from Abcam, lipopolysaccharide (LPS) (L4391) from Sigma-Aldrich, and rh Interferon (IFN)-γ (PHC4031) from Thermo Fisher Scientific.

    Techniques: Western Blot, Expressing, Derivative Assay, Control

    Western blot showing sarcomatoid-specific proteins (Met, CD44, ANXA2) and EMT markers (N-cadherin, Slug, Snail) in UOK-127 cells control, treated with M2-like macrophages, or treated with M2-like macrophages with siRNA knockdown of negative control or SPP1.

    Journal: bioRxiv

    Article Title: Spatial analysis reveals a novel inflammatory tumor transition state which promotes a macrophage-driven induction of sarcomatoid renal cell carcinoma

    doi: 10.1101/2025.06.12.656421

    Figure Lengend Snippet: Western blot showing sarcomatoid-specific proteins (Met, CD44, ANXA2) and EMT markers (N-cadherin, Slug, Snail) in UOK-127 cells control, treated with M2-like macrophages, or treated with M2-like macrophages with siRNA knockdown of negative control or SPP1.

    Article Snippet: We purchased Recombinant Human (rh) TGF-beta 1 (7754-BH), rh SPP1 (1433-OP), and rh CCL20 (360-MP) from R&D systems, rh Interleukin-4 (IL-4) (ab155733) from Abcam, lipopolysaccharide (LPS) (L4391) from Sigma-Aldrich, and rh Interferon (IFN)-γ (PHC4031) from Thermo Fisher Scientific.

    Techniques: Western Blot, Control, Knockdown, Negative Control

    Invasion assay of UOK-127 cell co-cultured with M2 macrophage conditioned media with negative controle (siNC) or silencing of SPP1 (siSPP1) in M2 macrophages.

    Journal: bioRxiv

    Article Title: Spatial analysis reveals a novel inflammatory tumor transition state which promotes a macrophage-driven induction of sarcomatoid renal cell carcinoma

    doi: 10.1101/2025.06.12.656421

    Figure Lengend Snippet: Invasion assay of UOK-127 cell co-cultured with M2 macrophage conditioned media with negative controle (siNC) or silencing of SPP1 (siSPP1) in M2 macrophages.

    Article Snippet: We purchased Recombinant Human (rh) TGF-beta 1 (7754-BH), rh SPP1 (1433-OP), and rh CCL20 (360-MP) from R&D systems, rh Interleukin-4 (IL-4) (ab155733) from Abcam, lipopolysaccharide (LPS) (L4391) from Sigma-Aldrich, and rh Interferon (IFN)-γ (PHC4031) from Thermo Fisher Scientific.

    Techniques: Invasion Assay, Cell Culture